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→About [http://www.genewiz.com/public/gene-synthesis.aspx GENEWIZ Gene Synthesis Services]
== About [http://www.genewiz.com/public/gene-synthesis.aspx GENEWIZ Gene Synthesis Services] ==
== About [http://www.genewiz.com/public/gene-synthesis.aspx GENEWIZ Gene Synthesis Services] ==
Gene synthesis is an excellent alternative to molecular cloning for custom construct production. Other important applications for de novo gene synthesis include the development of DNA vaccines, vectors for gene therapy, and gene libraries for protein engineering and directed molecular evolution.
[http://www.genewiz.com/public/gene-synthesis.aspx Gene Synthesis] is an excellent alternative to molecular cloning for custom construct production. Other important applications for de novo gene synthesis include the development of DNA vaccines, vectors for gene therapy, and gene libraries for protein engineering and directed molecular evolution.
'''ALGORITHMS FOR GENE SYNTHESIS''': DNA sequences which contain codon frequencies that differ significantly from the vectors usage bias will reduce translation efficiency of the protein and negatively impact expression levels. Thus, optimizing codon choice in the design of a synthetic gene is a critical factor in successful protein expression.
'''ALGORITHMS FOR GENE SYNTHESIS''': DNA sequences which contain codon frequencies that differ significantly from the vectors usage bias will reduce translation efficiency of the protein and negatively impact expression levels. Thus, optimizing codon choice in the design of a synthetic gene is a critical factor in successful protein expression.
''Oligo Design Algorithms'':
''Oligo Design Algorithms'':
• Minimize synthesis and assembly errors.
• Minimize synthesis and assembly errors
• Reduce variance in oligo melting temperature
• Reduce variance in oligo melting temperature
'''Result''': Reliably designed oligos can be synthesized and assembled into the desired target gene
''Codon Optimization Algorithms'':
''Codon Optimization Algorithms'':
• Match codon frequency in the desired sequence with the usage bias of the vector
• Match codon frequency in the desired sequence with the usage bias of the vector
• Optimize genes to prevent transcribed mRNAs from forming pesky secondary structures
• Optimize genes to prevent transcribed mRNAs from forming pesky secondary structures
• Satisfy your specific requirements for restriction sites.
• Satisfy your specific requirements for restriction sites
• Minimize occurrences of undesirable motifs and maximize occurrences of desirable motifs (For example: CpG motifs for immuno-modulation, cryptic splice sites for splicing).
• Minimize occurrences of undesirable motifs and maximize occurrences of desirable motifs (For example: CpG motifs for immuno-modulation, cryptic splice sites for splicing)
• Consider essential parameters including GC content, repeat sequences, mRNA instability motifs, inhibition sites, premature PolyA sites and so on
• Consider essential parameters including GC content, repeat sequences, mRNA instability motifs, inhibition sites, premature PolyA sites and so on
Result: Enhance target protein expression in a host cell
'''Result''': Enhance target protein expression in a host cell
== Why Gene Synthesis is better than the traditional cloning? ==
== Why Gene Synthesis is better than the traditional cloning? ==