11
edits
Changes
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• Minimize synthesis and assembly errors
• Minimize synthesis and assembly errors
• Reduce variance in oligo melting temperature
• Reduce variance in oligo melting temperature
'''Result''': Reliably designed oligos can be synthesized and assembled into the desired target gene
'''Result''': Reliably designed oligos can be synthesized and assembled into the desired target gene
• Minimize occurrences of undesirable motifs and maximize occurrences of desirable motifs (For example: CpG motifs for immuno-modulation, cryptic splice sites for splicing)
• Minimize occurrences of undesirable motifs and maximize occurrences of desirable motifs (For example: CpG motifs for immuno-modulation, cryptic splice sites for splicing)
• Consider essential parameters including GC content, repeat sequences, mRNA instability motifs, inhibition sites, premature PolyA sites and so on
• Consider essential parameters including GC content, repeat sequences, mRNA instability motifs, inhibition sites, premature PolyA sites and so on
'''Result''': Enhance target protein expression in a host cell
'''Result''': Enhance target protein expression in a host cell