MyWikiBiz, Author Your Legacy — Sunday October 06, 2024
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, 21:20, 16 February 2010
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| • Minimize synthesis and assembly errors | | • Minimize synthesis and assembly errors |
| • Reduce variance in oligo melting temperature | | • Reduce variance in oligo melting temperature |
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| '''Result''': Reliably designed oligos can be synthesized and assembled into the desired target gene | | '''Result''': Reliably designed oligos can be synthesized and assembled into the desired target gene |
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| • Minimize occurrences of undesirable motifs and maximize occurrences of desirable motifs (For example: CpG motifs for immuno-modulation, cryptic splice sites for splicing) | | • Minimize occurrences of undesirable motifs and maximize occurrences of desirable motifs (For example: CpG motifs for immuno-modulation, cryptic splice sites for splicing) |
| • Consider essential parameters including GC content, repeat sequences, mRNA instability motifs, inhibition sites, premature PolyA sites and so on | | • Consider essential parameters including GC content, repeat sequences, mRNA instability motifs, inhibition sites, premature PolyA sites and so on |
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| '''Result''': Enhance target protein expression in a host cell | | '''Result''': Enhance target protein expression in a host cell |
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