Changes

Gene synthesis is an excellent alternative to molecular cloning for custom construct production. Other important applications for de novo gene synthesis include the development of DNA vaccines, vectors for gene therapy, and gene libraries for protein engineering and directed molecular evolution.

Gene synthesis is an excellent alternative to molecular cloning for custom construct production. Other important applications for de novo gene synthesis include the development of DNA vaccines, vectors for gene therapy, and gene libraries for protein engineering and directed molecular evolution.

A'''LGORITHMS FOR GENE SYNTHESIS''': DNA sequences which contain codon frequencies that differ significantly from the vectors usage bias will reduce translation efficiency of the protein and negatively impact expression levels. Thus, optimizing codon choice in the design of a synthetic gene is a critical factor in successful protein expression.  

'''ALGORITHMS FOR GENE SYNTHESIS''': DNA sequences which contain codon frequencies that differ significantly from the vectors usage bias will reduce translation efficiency of the protein and negatively impact expression levels. Thus, optimizing codon choice in the design of a synthetic gene is a critical factor in successful protein expression.  

''Oligo Design Algorithms'':  

''Oligo Design Algorithms'':  

• Consider essential parameters including GC content, repeat sequences, mRNA instability motifs, inhibition sites, premature PolyA sites and so on

• Consider essential parameters including GC content, repeat sequences, mRNA instability motifs, inhibition sites, premature PolyA sites and so on

Result: Enhance target protein expression in a host cell

Result: Enhance target protein expression in a host cell

== Why Gene Synthesis is better than the traditional cloning? ==

== Why Gene Synthesis is better than the traditional cloning? ==